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Elabscience
SKU:E-EL-H6066
Human HIF-1α(Hypoxia Inducible Factor 1 Alpha) ELISA Kit
Human HIF-1α(Hypoxia Inducible Factor 1 Alpha) ELISA Kit
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$495.00 USD
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The Human HIF-1α (Hypoxia Inducible Factor 1 Alpha) ELISA Kit is a cutting-edge and highly reliable diagnostic tool designed for the accurate detection and quantification of HIF-1α in human samples. This kit is specifically developed to cater to the needs of researchers and clinicians involved in the study and diagnosis of hypoxia-related diseases and conditions.
Utilizing the enzyme-linked immunosorbent assay (ELISA) technique, this kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results. The assay is based on the principle of a solid-phase sandwich enzyme immunoassay, where HIF-1α present in the sample is captured by a specific antibody coated on the microplate wells. Subsequently, a detection antibody conjugated with horseradish peroxidase (HRP) is added, followed by the addition of a substrate solution. The reaction between the HRP and substrate leads to the development of a colored product, the intensity of which is directly proportional to the concentration of HIF-1α in the sample.
This ELISA kit provides a wide dynamic range, allowing for the accurate measurement of HIF-1α levels in various sample types, including serum, plasma, and cell culture supernatants. With its user-friendly protocol and comprehensive instructions, this kit offers convenience and efficiency, making it an indispensable tool for researchers and clinicians in the field of hypoxia research and diagnosis.
In summary, the Human HIF-1α ELISA Kit is a state-of-the-art diagnostic tool that offers exceptional sensitivity, specificity, and accuracy in the detection and quantification of HIF-1α. Its ease of use and reliable performance make it an invaluable asset for researchers and clinicians working in the field of hypoxia-related diseases and conditions.
Utilizing the enzyme-linked immunosorbent assay (ELISA) technique, this kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results. The assay is based on the principle of a solid-phase sandwich enzyme immunoassay, where HIF-1α present in the sample is captured by a specific antibody coated on the microplate wells. Subsequently, a detection antibody conjugated with horseradish peroxidase (HRP) is added, followed by the addition of a substrate solution. The reaction between the HRP and substrate leads to the development of a colored product, the intensity of which is directly proportional to the concentration of HIF-1α in the sample.
This ELISA kit provides a wide dynamic range, allowing for the accurate measurement of HIF-1α levels in various sample types, including serum, plasma, and cell culture supernatants. With its user-friendly protocol and comprehensive instructions, this kit offers convenience and efficiency, making it an indispensable tool for researchers and clinicians in the field of hypoxia research and diagnosis.
In summary, the Human HIF-1α ELISA Kit is a state-of-the-art diagnostic tool that offers exceptional sensitivity, specificity, and accuracy in the detection and quantification of HIF-1α. Its ease of use and reliable performance make it an invaluable asset for researchers and clinicians working in the field of hypoxia-related diseases and conditions.
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