Human Irisin ELISA Kit

The Human Irisin ELISA Kit is a highly sensitive and specific assay designed for the quantitative detection of human irisin in serum, plasma, and cell culture supernatants. This kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) format, which allows for the accurate and precise measurement of irisin levels in biological samples. The assay is based on the principle of a monoclonal antibody specific for human irisin, which is coated onto a microplate.

The sample is added to the microplate, and any irisin present in the sample binds to the immobilized antibody. A second biotinylated monoclonal antibody specific for irisin is then added, followed by streptavidin-HRP conjugate. The resulting complex is detected by the addition of a chromogenic substrate, and the absorbance is measured at 450 nm. The Human Irisin ELISA Kit is a reliable and reproducible tool for the detection of irisin, and is suitable for use in research and clinical applications. It offers a simple and cost-effective solution for the measurement of irisin levels in a variety of biological samples.