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SKU:E-EL-H6125
Human IFNα(Interferon Alpha) ELISA Kit
Human IFNα(Interferon Alpha) ELISA Kit
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$495.00 USD
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The HUMAN IFNΑ (Interferon Alpha) ELISA Kit is a highly reliable and accurate diagnostic tool designed for the detection of Interferon Alpha in human serum, plasma, and cell culture supernatants. This kit is intended for research purposes only and is not intended for diagnostic or therapeutic use.
The kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) technique, which utilizes monoclonal antibodies specific to human IFNΑ. The assay is performed in a 96-well microplate coated with a monoclonal antibody specific to human IFNΑ. The sample is added to the microplate and incubated, allowing the IFNΑ to bind to the immobilized antibody. After washing, a biotinylated monoclonal antibody specific to human IFNΑ is added, followed by streptavidin-horseradish peroxidase conjugate. The resulting complex is detected by the addition of a chromogenic substrate, and the absorbance is measured at 450 nm.
The HUMAN IFNΑ ELISA Kit is highly sensitive, with a detection range of 15.6-1000 pg/mL. The kit has a high degree of specificity, with no cross-reactivity with other cytokines or growth factors. The kit is easy to use, with a simple protocol that can be completed in less than 4 hours.
In conclusion, the HUMAN IFNΑ ELISA Kit is a reliable and accurate diagnostic tool for the detection of Interferon Alpha in human serum, plasma, and cell culture supernatants. It is an essential tool for researchers studying the role of IFNΑ in various diseases and conditions.
The kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) technique, which utilizes monoclonal antibodies specific to human IFNΑ. The assay is performed in a 96-well microplate coated with a monoclonal antibody specific to human IFNΑ. The sample is added to the microplate and incubated, allowing the IFNΑ to bind to the immobilized antibody. After washing, a biotinylated monoclonal antibody specific to human IFNΑ is added, followed by streptavidin-horseradish peroxidase conjugate. The resulting complex is detected by the addition of a chromogenic substrate, and the absorbance is measured at 450 nm.
The HUMAN IFNΑ ELISA Kit is highly sensitive, with a detection range of 15.6-1000 pg/mL. The kit has a high degree of specificity, with no cross-reactivity with other cytokines or growth factors. The kit is easy to use, with a simple protocol that can be completed in less than 4 hours.
In conclusion, the HUMAN IFNΑ ELISA Kit is a reliable and accurate diagnostic tool for the detection of Interferon Alpha in human serum, plasma, and cell culture supernatants. It is an essential tool for researchers studying the role of IFNΑ in various diseases and conditions.
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