Human IL-6(Interleukin 6)  ELISA Kit

The Human IL-6 (Interleukin 6) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of human IL-6 in serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of the target protein. The assay is performed in a 96-well microplate coated with a monoclonal antibody against human IL-6.

The sample is added to the wells, and the target protein is captured by the immobilized antibody. After washing away unbound substances, a biotinylated polyclonal antibody against human IL-6 is added, followed by streptavidin-conjugated horseradish peroxidase (HRP). The HRP catalyzes the conversion of a colorless substrate to a colored product, which is proportional to the amount of human IL-6 present in the sample. The absorbance is measured at 450 nm using a microplate reader. This kit has a detection range of 7.8-500 pg/mL and a sensitivity of 2.0 pg/mL. It is suitable for research use in various fields, including immunology, oncology, and inflammation.