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Mouse FGF21(Fibroblast Growth Factor 21) ELISA Kit
Mouse FGF21(Fibroblast Growth Factor 21) ELISA Kit
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The Mouse FGF21 ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of Fibroblast Growth Factor 21 (FGF21) in mouse serum, plasma, and cell culture supernatants. FGF21 is a hormone-like protein that plays a crucial role in regulating glucose and lipid metabolism, as well as energy homeostasis. Dysregulation of FGF21 has been implicated in a variety of metabolic disorders, including obesity, type 2 diabetes, and non-alcoholic fatty liver disease.
The Mouse FGF21 ELISA Kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) format, in which a monoclonal antibody specific to mouse FGF21 is coated onto a microplate. Samples are added to the wells, and any FGF21 present in the sample binds to the immobilized antibody. A biotinylated polyclonal antibody specific to mouse FGF21 is then added, followed by streptavidin-conjugated horseradish peroxidase (HRP). The HRP catalyzes a colorimetric reaction, which is detected by measuring the absorbance at 450 nm. The intensity of the color is directly proportional to the amount of FGF21 present in the sample.
The Mouse FGF21 ELISA Kit is a reliable and accurate tool for the quantitative measurement of FGF21 in mouse samples. It has a detection range of 15.6-1000 pg/mL, with a sensitivity of 7.8 pg/mL. The kit is easy to use, with a simple protocol that can be completed in less than 4 hours. It is suitable for use in a variety of research applications, including metabolic studies, drug development, and biomarker discovery. The kit is manufactured to the highest quality standards, with rigorous quality control measures in place to ensure reproducibility and accuracy.
The Mouse FGF21 ELISA Kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) format, in which a monoclonal antibody specific to mouse FGF21 is coated onto a microplate. Samples are added to the wells, and any FGF21 present in the sample binds to the immobilized antibody. A biotinylated polyclonal antibody specific to mouse FGF21 is then added, followed by streptavidin-conjugated horseradish peroxidase (HRP). The HRP catalyzes a colorimetric reaction, which is detected by measuring the absorbance at 450 nm. The intensity of the color is directly proportional to the amount of FGF21 present in the sample.
The Mouse FGF21 ELISA Kit is a reliable and accurate tool for the quantitative measurement of FGF21 in mouse samples. It has a detection range of 15.6-1000 pg/mL, with a sensitivity of 7.8 pg/mL. The kit is easy to use, with a simple protocol that can be completed in less than 4 hours. It is suitable for use in a variety of research applications, including metabolic studies, drug development, and biomarker discovery. The kit is manufactured to the highest quality standards, with rigorous quality control measures in place to ensure reproducibility and accuracy.
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