Mouse HIF-1α(Hypoxia Inducible Factor 1 Alpha) ELISA Kit

The Mouse HIF-1α (Hypoxia Inducible Factor 1 Alpha) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of HIF-1α in mouse serum, plasma, and tissue homogenates. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) technique, which utilizes two specific antibodies that bind to different epitopes of HIF-1α. The captured HIF-1α is then detected using a biotinylated antibody and streptavidin-HRP conjugate, followed by the addition of a chromogenic substrate that produces a colorimetric signal proportional to the amount of HIF-1α present in the sample.

This ELISA kit has a wide dynamic range of detection, with a lower limit of detection of 0.156 ng/mL and an upper limit of 10 ng/mL. It has excellent specificity and sensitivity, with minimal cross-reactivity with other related proteins. The kit is easy to use, with a simple protocol that can be completed within 3 hours. It is suitable for use in research applications, including the study of hypoxia-induced signaling pathways, cancer biology, and cardiovascular disease. Overall, the Mouse HIF-1α ELISA Kit is a valuable tool for the accurate and precise quantification of HIF-1α in mouse samples.