Mouse Fcγ(Fc Fragment of IgG) ELISA Kit

The Mouse Fcγ (Fc Fragment of IgG) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative measurement of Fcγ in mouse serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes a monoclonal antibody specific to mouse Fcγ as the capture antibody and a biotinylated polyclonal antibody as the detection antibody.

The assay is performed in a 96-well microplate coated with the capture antibody, and the detection antibody is added to the wells after the sample and standards have been added. The biotinylated detection antibody binds to the captured Fcγ, and the signal is amplified by the addition of streptavidin-HRP conjugate and TMB substrate. The absorbance is measured at 450 nm, and the concentration of Fcγ in the sample is determined by comparing the absorbance values to a standard curve. This kit is highly specific, with minimal cross-reactivity with other mouse proteins, and has a detection range of 0.156-10 ng/mL. It is a valuable tool for researchers studying the role of Fcγ in immune responses and disease pathogenesis.