Mouse IL-35(Interleukin 35) ELISA Kit

The Mouse IL-35 (Interleukin 35) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of mouse IL-35 in serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of the target protein. The assay is performed in a 96-well microplate coated with a monoclonal antibody against mouse IL-35. The sample is added to the wells, and the target protein is captured by the immobilized antibody.

A biotinylated polyclonal antibody against mouse IL-35 is then added, followed by streptavidin-conjugated horseradish peroxidase (HRP). The HRP catalyzes the conversion of the chromogenic substrate, resulting in a color change that is proportional to the amount of mouse IL-35 present in the sample. The Mouse IL-35 ELISA Kit has a detection range of 15.6-1000 pg/mL and a sensitivity of 9.4 pg/mL. This kit is easy to use, highly reproducible, and provides accurate and precise results, making it an ideal tool for researchers studying the role of IL-35 in various biological processes.