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Elabscience
SKU:E-EL-M0780
Mouse MMP-2(Matrix Metalloproteinase 2) ELISA Kit
Mouse MMP-2(Matrix Metalloproteinase 2) ELISA Kit
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$495.00 USD
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The Mouse MMP-2 ELISA Kit is specifically designed for the detection of MMP-2 in mouse samples, making it a reliable and sensitive tool for researchers studying the role of this protein in various biological processes. MMP-2, also known as gelatinase A, is a member of the matrix metalloproteinase family, which plays a crucial role in tissue remodeling, angiogenesis, and cancer progression.
The kit utilizes the sandwich ELISA principle, which involves the use of a monoclonal antibody specific for MMP-2 to capture the target protein. This antibody is pre-coated on microplates provided in the kit. After the target protein is captured, a biotinylated polyclonal antibody is added to detect the captured protein. Streptavidin-HRP is then added to bind to the biotinylated antibody, forming a complex that can be detected using a colorimetric reaction with the TMB substrate. The reaction is stopped with the stop solution, and the absorbance is measured using a microplate reader.
The Mouse MMP-2 ELISA Kit includes all necessary reagents and components for the assay, ensuring convenience and ease of use. The pre-coated microplates eliminate the need for additional coating steps, saving time and effort. The kit also provides standards of known MMP-2 concentrations, allowing for the quantification of the target protein in the samples.
The assay can be completed in approximately 3 hours, providing quick results for researchers. The detection range of the kit is 0.156-10 ng/mL, with a sensitivity of 0.078 ng/mL. This wide detection range and high sensitivity make the kit suitable for detecting MMP-2 in a variety of sample types, including serum, plasma, and cell culture supernatants.
Overall, the Mouse MMP-2 ELISA Kit is a valuable tool for researchers studying the role of MMP-2 in various biological processes. Its reliability, sensitivity, and convenience make it an ideal choice for quantitative detection of MMP-2 in mouse samples. By providing accurate and reproducible results, this kit contributes to the advancement of research in tissue remodeling, angiogenesis, and cancer progression.
The kit utilizes the sandwich ELISA principle, which involves the use of a monoclonal antibody specific for MMP-2 to capture the target protein. This antibody is pre-coated on microplates provided in the kit. After the target protein is captured, a biotinylated polyclonal antibody is added to detect the captured protein. Streptavidin-HRP is then added to bind to the biotinylated antibody, forming a complex that can be detected using a colorimetric reaction with the TMB substrate. The reaction is stopped with the stop solution, and the absorbance is measured using a microplate reader.
The Mouse MMP-2 ELISA Kit includes all necessary reagents and components for the assay, ensuring convenience and ease of use. The pre-coated microplates eliminate the need for additional coating steps, saving time and effort. The kit also provides standards of known MMP-2 concentrations, allowing for the quantification of the target protein in the samples.
The assay can be completed in approximately 3 hours, providing quick results for researchers. The detection range of the kit is 0.156-10 ng/mL, with a sensitivity of 0.078 ng/mL. This wide detection range and high sensitivity make the kit suitable for detecting MMP-2 in a variety of sample types, including serum, plasma, and cell culture supernatants.
Overall, the Mouse MMP-2 ELISA Kit is a valuable tool for researchers studying the role of MMP-2 in various biological processes. Its reliability, sensitivity, and convenience make it an ideal choice for quantitative detection of MMP-2 in mouse samples. By providing accurate and reproducible results, this kit contributes to the advancement of research in tissue remodeling, angiogenesis, and cancer progression.
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