Mouse NUP35(Nucleoporin 35kDa) ELISA Kit

The Mouse NUP35 (Nucleoporin 35kDa) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of NUP35 in mouse serum, plasma, and other biological fluids. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of NUP35. The capture antibody is coated on a microplate, and the detection antibody is conjugated with horseradish peroxidase (HRP).

The sample is added to the microplate, and NUP35 in the sample binds to the capture antibody. After washing away any unbound substances, the detection antibody-HRP conjugate is added, which binds to the captured NUP35. The excess detection antibody-HRP conjugate is washed away, and a substrate solution is added, which reacts with the HRP to produce a colorimetric signal. The intensity of the signal is proportional to the amount of NUP35 present in the sample. This kit is easy to use, highly specific, and has a wide dynamic range, making it an ideal tool for researchers studying NUP35 in mouse models.