Mouse NUP98(Nucleoporin 98kDa) ELISA Kit

The Mouse NUP98 (Nucleoporin 98kDa) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of NUP98 in mouse serum, plasma, and other biological fluids. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of NUP98. The capture antibody is coated onto the microplate wells, while the detection antibody is conjugated to horseradish peroxidase (HRP). The sample is added to the wells, and NUP98 in the sample binds to the capture antibody.

After washing away any unbound substances, the HRP-conjugated detection antibody is added, which binds to the captured NUP98. The excess detection antibody is washed away, and a substrate solution is added, which reacts with the HRP to produce a color change. The intensity of the color is directly proportional to the concentration of NUP98 in the sample. This kit has a detection range of 0.156-10 ng/mL and a sensitivity of 0.094 ng/mL. It is a valuable tool for researchers studying the role of NUP98 in various biological processes and diseases.