Mouse NUP133(Nucleoporin 133kDa) ELISA Kit

The Mouse NUP133 (Nucleoporin 133kDa) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of NUP133 in mouse serum, plasma, and other biological fluids. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of NUP133. The capture antibody is coated onto a microplate, and the detection antibody is conjugated with horseradish peroxidase (HRP). The sample is added to the microplate, and NUP133 in the sample binds to the capture antibody.

After washing away any unbound substances, the HRP-conjugated detection antibody is added, which binds to the captured NUP133. The excess detection antibody is washed away, and a substrate solution is added, which reacts with the HRP enzyme to produce a colorimetric signal. The intensity of the signal is proportional to the amount of NUP133 present in the sample. This kit has a detection range of 0.156-10 ng/mL and a sensitivity of 0.094 ng/mL. It is a valuable tool for researchers studying the role of NUP133 in various biological processes and diseases.