Mouse tPA(Tissue-type Plasminogen Activator) ELISA Kit

The Mouse tPA (Tissue-type Plasminogen Activator) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of tPA in mouse serum, plasma, and other biological fluids. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of tPA. The capture antibody is coated on a microplate, while the detection antibody is conjugated with horseradish peroxidase (HRP).

The tPA in the sample binds to the capture antibody, and the detection antibody-HRP conjugate binds to the tPA, forming a sandwich complex. The addition of a substrate solution leads to the development of a blue color, which turns yellow upon the addition of a stop solution. The intensity of the color is proportional to the amount of tPA present in the sample. This kit has a detection range of 0.156-10 ng/mL, with a sensitivity of 0.078 ng/mL. It is easy to use, with a total assay time of 3 hours and 30 minutes. The Mouse tPA ELISA Kit is an essential tool for researchers studying the role of tPA in various physiological and pathological processes.