Mouse PDGF-D(Platelet Derived Growth Factor D) ELISA Kit

The Mouse PDGF-D (Platelet Derived Growth Factor D) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of PDGF-D in mouse serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes a monoclonal antibody specific to mouse PDGF-D coated onto a microplate. The sample is added to the microplate, and any PDGF-D present in the sample binds to the immobilized antibody. After washing away any unbound substances, a biotinylated monoclonal antibody specific to mouse PDGF-D is added to the microplate, which binds to the captured PDGF-D.

Following another washing step, streptavidin-conjugated horseradish peroxidase is added to the microplate, which binds to the biotinylated antibody. Finally, a substrate solution is added to the microplate, and the color development is measured at 450 nm. The Mouse PDGF-D ELISA Kit is a highly sensitive and specific assay that provides accurate and reproducible results, making it an ideal tool for researchers studying PDGF-D in mouse models.