Mouse PDGF-B(Platelet Derived Growth Factor Subunit B) ELISA Kit

The Mouse PDGF-B (Platelet Derived Growth Factor Subunit B) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of PDGF-B in mouse serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes a monoclonal antibody specific to mouse PDGF-B coated onto a microplate. The sample is added to the microplate, and any PDGF-B present in the sample binds to the immobilized antibody.

After washing away any unbound substances, a biotin-conjugated monoclonal antibody specific to mouse PDGF-B is added to the microplate. This antibody binds to the captured PDGF-B, forming a sandwich complex. Following another washing step, an enzyme-conjugated streptavidin is added to the microplate, which binds to the biotinylated antibody. Finally, a substrate solution is added, and the color development is measured at 450 nm. The Mouse PDGF-B ELISA Kit is a highly sensitive and specific assay, with a detection range of 15.6-1000 pg/mL. This kit is suitable for research purposes and is intended for in vitro use only.