Mouse PTN(Pleiotrophin) ELISA Kit

The Mouse PTN (Pleiotrophin) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of mouse PTN in serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of the target protein. The assay is performed in a 96-well microplate coated with a monoclonal antibody against mouse PTN.

The sample is added to the wells, and the PTN in the sample binds to the immobilized antibody. After washing away any unbound substances, a biotinylated polyclonal antibody against mouse PTN is added, which binds to the captured PTN. Streptavidin-HRP conjugate is then added, which binds to the biotinylated antibody. Finally, a substrate solution is added, and the color development is measured at 450 nm. The Mouse PTN ELISA Kit has a detection range of 0.156-10 ng/mL, with a sensitivity of 0.078 ng/mL. This kit is easy to use, highly reproducible, and provides accurate and precise results, making it an ideal tool for researchers studying the role of PTN in various biological processes.