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Elabscience

SKU:E-EL-M0951

Mouse PCNA(Proliferating Cell Nuclear Antigen) ELISA Kit

Mouse PCNA(Proliferating Cell Nuclear Antigen) ELISA Kit

Regular price $609.00 USD
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The Mouse PCNA (Proliferating Cell Nuclear Antigen) ELISA Kit is a highly reliable and efficient tool designed for the quantitative detection of PCNA in mouse samples. PCNA is a crucial protein involved in DNA replication and repair, making it an essential marker for cell proliferation and DNA synthesis studies.

This ELISA kit utilizes a sandwich enzyme immunoassay technique, ensuring accurate and sensitive measurement of PCNA levels in a wide range of sample types, including serum, plasma, and tissue homogenates. The assay is based on the principle of specific antigen-antibody interaction, where PCNA in the sample binds to the immobilized anti-PCNA antibody on the microplate wells. After a series of washing steps to remove any unbound substances, a horseradish peroxidase (HRP)-conjugated anti-PCNA antibody is added, forming an immune complex. The addition of a chromogenic substrate triggers a color change, which is directly proportional to the PCNA concentration in the sample.

This Mouse PCNA ELISA Kit offers excellent sensitivity, with a detection limit as low as 0.1 ng/mL. It also exhibits high specificity, ensuring minimal cross-reactivity with other related proteins. The kit provides a user-friendly protocol, with clear instructions and all necessary reagents included. With its superior performance and ease of use, this ELISA kit is an indispensable tool for researchers studying cell proliferation, DNA replication, and related fields in mouse models.
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