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Elabscience
SKU:E-EL-M2405
Mouse αFP(Alpha-Fetoprotein) ELISA Kit
Mouse αFP(Alpha-Fetoprotein) ELISA Kit
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$609.00 USD
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Alpha-Fetoprotein (AFP) is a glycoprotein that is produced during fetal development and is normally found in low levels in adult serum. However, elevated levels of AFP in adult serum can be indicative of certain types of cancer, particularly hepatocellular carcinoma (HCC), as well as other liver diseases and germ cell tumors. The Mouse αFP ELISA Kit provides a highly sensitive and specific method for detecting AFP in mouse serum, plasma, and other biological fluids, making it an essential tool for researchers and clinicians working in the field of cancer diagnosis and treatment.
The kit is based on the sandwich ELISA technique, which involves the use of two highly specific monoclonal antibodies that bind to different epitopes on the AFP molecule. The first antibody is immobilized on the surface of a microplate, while the second antibody is conjugated to an enzyme, such as horseradish peroxidase (HRP). When a sample containing AFP is added to the microplate, the first antibody captures the AFP molecule, while the second antibody-HRP conjugate binds to a different epitope on the AFP molecule. After washing away any unbound material, a substrate solution is added to the microplate, which reacts with the HRP enzyme to produce a colorimetric signal that is proportional to the amount of AFP present in the sample.
The Mouse αFP ELISA Kit is easy to use and provides rapid results, with a total assay time of less than 3 hours. The kit has a detection range of 0.156-10 ng/mL, with a sensitivity of 0.078 ng/mL. The kit has been validated for use with mouse serum, plasma, and other biological fluids, and has been shown to have excellent precision and accuracy. The kit is manufactured to the highest quality standards and is CE certified, ensuring consistent and reliable performance.
In conclusion, the Mouse αFP ELISA Kit is a highly reliable and accurate diagnostic tool for the quantitative detection of AFP in mouse serum, plasma, and other biological fluids. It is an essential tool for researchers and clinicians working in the field of cancer diagnosis and treatment, particularly for the detection of HCC and other liver diseases. The kit is easy to use, provides rapid results, and is manufactured to the highest quality standards, ensuring consistent and reliable performance.
The kit is based on the sandwich ELISA technique, which involves the use of two highly specific monoclonal antibodies that bind to different epitopes on the AFP molecule. The first antibody is immobilized on the surface of a microplate, while the second antibody is conjugated to an enzyme, such as horseradish peroxidase (HRP). When a sample containing AFP is added to the microplate, the first antibody captures the AFP molecule, while the second antibody-HRP conjugate binds to a different epitope on the AFP molecule. After washing away any unbound material, a substrate solution is added to the microplate, which reacts with the HRP enzyme to produce a colorimetric signal that is proportional to the amount of AFP present in the sample.
The Mouse αFP ELISA Kit is easy to use and provides rapid results, with a total assay time of less than 3 hours. The kit has a detection range of 0.156-10 ng/mL, with a sensitivity of 0.078 ng/mL. The kit has been validated for use with mouse serum, plasma, and other biological fluids, and has been shown to have excellent precision and accuracy. The kit is manufactured to the highest quality standards and is CE certified, ensuring consistent and reliable performance.
In conclusion, the Mouse αFP ELISA Kit is a highly reliable and accurate diagnostic tool for the quantitative detection of AFP in mouse serum, plasma, and other biological fluids. It is an essential tool for researchers and clinicians working in the field of cancer diagnosis and treatment, particularly for the detection of HCC and other liver diseases. The kit is easy to use, provides rapid results, and is manufactured to the highest quality standards, ensuring consistent and reliable performance.
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