Elabscience
SKU:E-EL-M2567
Mouse CYP2E1(Cytochrome P450, family 2, subfamily E, polypeptide 1) ELISA Kit
Mouse CYP2E1(Cytochrome P450, family 2, subfamily E, polypeptide 1) ELISA Kit
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The Mouse CYP2E1 (Cytochrome P450, family 2, subfamily E, polypeptide 1) ELISA Kit is a highly reliable and accurate diagnostic tool designed for the quantitative detection of CYP2E1 in mouse serum, plasma, and other biological samples. This kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique, which employs specific antibodies to capture and detect CYP2E1 in the sample. The kit is easy to use, with a simple and straightforward protocol that requires minimal sample preparation. The Mouse CYP2E1 ELISA Kit is suitable for use in research and clinical laboratories, and it provides a rapid and cost-effective means of measuring CYP2E1 levels in mouse samples. The kit is manufactured to the highest quality standards, ensuring consistent and reproducible results. It is an essential tool for researchers and clinicians working in the field of drug metabolism and toxicology, and as well as those studying the role of CYP2E1 in various disease states.
The MOUSE CYP2E1 (Cytochrome P450, Family 2, Subfamily E, Polypeptide 1) ELISA Kit is a highly reliable and accurate tool designed for the quantitative measurement of CYP2E1 levels in mouse samples. This enzyme plays a crucial role in the metabolism of various xenobiotics and endogenous compounds, making it an essential target for research in drug metabolism, toxicology, and environmental health.
This ELISA kit utilizes a sandwich enzyme immunoassay technique, employing specific antibodies that recognize and bind to the CYP2E1 protein. The assay is performed in a 96-well microplate, coated with a capture antibody that selectively immobilizes the target protein. Following sample addition and incubation, a detection antibody conjugated with horseradish peroxidase is introduced, forming an immune complex with the captured CYP2E1. Subsequent addition of a chromogenic substrate leads to the development of a colorimetric signal, whose intensity is directly proportional to the concentration of CYP2E1 in the sample.
The MOUSE CYP2E1 ELISA Kit offers exceptional sensitivity, with a detection range of 0.156-10 ng/mL, allowing for precise quantification of CYP2E1 levels even in low abundance samples. The assay exhibits excellent specificity, ensuring minimal cross-reactivity with other related proteins. Furthermore, the kit provides a high degree of reproducibility, with intra-assay and inter-assay coefficients of variation less than 10% and 12%, respectively.
This ELISA kit is user-friendly, with a straightforward protocol that can be completed within a few hours. It includes all necessary reagents and controls, ensuring accurate and reliable results. The kit is suitable for use in various research settings, including academic laboratories, pharmaceutical companies, and biotechnology research institutions.
In summary, the MOUSE CYP2E1 ELISA Kit is a valuable tool for researchers aiming to investigate the role of CYP2E1 in drug metabolism, toxicology, and environmental health. Its high sensitivity, specificity, and reproducibility make it an indispensable asset in the field of molecular biology and biomedical research.
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