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Elabscience
SKU:E-EL-M2701
Mouse TFAM(Transcription Factor A, Mitochondrial) ELISA Kit
Mouse TFAM(Transcription Factor A, Mitochondrial) ELISA Kit
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$609.00 USD
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The Mouse TFAM (Transcription Factor A, Mitochondrial) ELISA Kit is a highly reliable and accurate tool designed for the quantitative measurement of TFAM levels in mouse samples. TFAM is a crucial protein involved in the regulation of mitochondrial DNA replication and transcription, making it an essential factor in maintaining mitochondrial function and overall cellular homeostasis.
This ELISA kit utilizes a sandwich enzyme immunoassay technique, ensuring high sensitivity and specificity in detecting TFAM. The assay is based on the principle of antigen-antibody interaction, where TFAM in the sample binds to the pre-coated capture antibody. After a series of washing steps to remove any unbound substances, a detection antibody specific to TFAM is added, forming a sandwich complex. This complex is then visualized using a substrate solution, generating a colorimetric signal that is directly proportional to the concentration of TFAM in the sample.
The Mouse TFAM ELISA Kit offers several advantages, including its wide dynamic range, low sample volume requirement, and rapid assay time. With a detection range of 0.156-10 ng/mL, this kit allows for the accurate quantification of TFAM levels in a variety of mouse samples, including serum, plasma, and tissue homogenates. Additionally, the kit requires only a small sample volume, minimizing the need for sample collection and conserving valuable biological material. The entire assay can be completed within 3 hours, enabling efficient and timely analysis of TFAM levels.
This ELISA kit has been extensively validated and optimized to ensure reliable and reproducible results. It exhibits excellent intra-assay and inter-assay precision, with coefficients of variation less than 10%. Furthermore, the kit demonstrates high specificity, showing minimal cross-reactivity with other related proteins.
In summary, the Mouse TFAM ELISA Kit is a valuable tool for researchers studying mitochondrial function and its role in various biological processes. Its high sensitivity, specificity, and ease of use make it an ideal choice for accurately quantifying TFAM levels in mouse samples.
This ELISA kit utilizes a sandwich enzyme immunoassay technique, ensuring high sensitivity and specificity in detecting TFAM. The assay is based on the principle of antigen-antibody interaction, where TFAM in the sample binds to the pre-coated capture antibody. After a series of washing steps to remove any unbound substances, a detection antibody specific to TFAM is added, forming a sandwich complex. This complex is then visualized using a substrate solution, generating a colorimetric signal that is directly proportional to the concentration of TFAM in the sample.
The Mouse TFAM ELISA Kit offers several advantages, including its wide dynamic range, low sample volume requirement, and rapid assay time. With a detection range of 0.156-10 ng/mL, this kit allows for the accurate quantification of TFAM levels in a variety of mouse samples, including serum, plasma, and tissue homogenates. Additionally, the kit requires only a small sample volume, minimizing the need for sample collection and conserving valuable biological material. The entire assay can be completed within 3 hours, enabling efficient and timely analysis of TFAM levels.
This ELISA kit has been extensively validated and optimized to ensure reliable and reproducible results. It exhibits excellent intra-assay and inter-assay precision, with coefficients of variation less than 10%. Furthermore, the kit demonstrates high specificity, showing minimal cross-reactivity with other related proteins.
In summary, the Mouse TFAM ELISA Kit is a valuable tool for researchers studying mitochondrial function and its role in various biological processes. Its high sensitivity, specificity, and ease of use make it an ideal choice for accurately quantifying TFAM levels in mouse samples.
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