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SKU:E-EL-M3006
Mouse IGF-1(Insulin-like Growth Factor 1) ELISA Kit
Mouse IGF-1(Insulin-like Growth Factor 1) ELISA Kit
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$609.00 USD
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The Mouse IGF-1 (Insulin-like Growth Factor 1) ELISA Kit is a highly reliable and accurate tool designed for the quantitative measurement of IGF-1 levels in mouse serum, plasma, and other biological samples. This kit utilizes a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA) technique, ensuring precise and sensitive detection of IGF-1.
The Mouse IGF-1 ELISA Kit is composed of pre-coated microplate wells, ready-to-use standards, controls, and all necessary reagents. The assay procedure involves the addition of samples and standards to the microplate wells, followed by the addition of a biotin-conjugated IGF-1 antibody. After incubation and washing steps, a streptavidin-HRP conjugate is added, which binds to the biotinylated antibody. Subsequent addition of the substrate solution leads to the development of a blue color, which turns yellow after the addition of the stop solution. The intensity of the color is directly proportional to the concentration of IGF-1 in the samples.
This Mouse IGF-1 ELISA Kit offers a wide dynamic range of detection, allowing for the quantification of IGF-1 levels ranging from 0.156 ng/mL to 10 ng/mL. The sensitivity of this kit is 0.078 ng/mL, ensuring accurate measurement even at low concentrations. The intra-assay and inter-assay precision coefficients of variation (CVs) are less than 10% and 12%, respectively, guaranteeing reproducible and reliable results.
The Mouse IGF-1 ELISA Kit is user-friendly and time-efficient, with a total assay time of approximately 3 hours. It provides high specificity, as it does not cross-react with other related proteins. This kit is suitable for research purposes in various fields, including endocrinology, growth factor studies, and developmental biology.
In summary, the Mouse IGF-1 ELISA Kit is a robust and precise tool for the quantitative measurement of IGF-1 levels in mouse samples. Its high sensitivity, wide dynamic range, and user-friendly design make it an ideal choice for researchers seeking accurate and reliable results in their studies.
The Mouse IGF-1 ELISA Kit is composed of pre-coated microplate wells, ready-to-use standards, controls, and all necessary reagents. The assay procedure involves the addition of samples and standards to the microplate wells, followed by the addition of a biotin-conjugated IGF-1 antibody. After incubation and washing steps, a streptavidin-HRP conjugate is added, which binds to the biotinylated antibody. Subsequent addition of the substrate solution leads to the development of a blue color, which turns yellow after the addition of the stop solution. The intensity of the color is directly proportional to the concentration of IGF-1 in the samples.
This Mouse IGF-1 ELISA Kit offers a wide dynamic range of detection, allowing for the quantification of IGF-1 levels ranging from 0.156 ng/mL to 10 ng/mL. The sensitivity of this kit is 0.078 ng/mL, ensuring accurate measurement even at low concentrations. The intra-assay and inter-assay precision coefficients of variation (CVs) are less than 10% and 12%, respectively, guaranteeing reproducible and reliable results.
The Mouse IGF-1 ELISA Kit is user-friendly and time-efficient, with a total assay time of approximately 3 hours. It provides high specificity, as it does not cross-react with other related proteins. This kit is suitable for research purposes in various fields, including endocrinology, growth factor studies, and developmental biology.
In summary, the Mouse IGF-1 ELISA Kit is a robust and precise tool for the quantitative measurement of IGF-1 levels in mouse samples. Its high sensitivity, wide dynamic range, and user-friendly design make it an ideal choice for researchers seeking accurate and reliable results in their studies.
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