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Elabscience
SKU:E-EL-M3030
Mouse OPN(Osteopontin) ELISA Kit
Mouse OPN(Osteopontin) ELISA Kit
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$609.00 USD
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The Mouse OPN (Osteopontin) ELISA Kit is a highly reliable and accurate diagnostic tool designed for the quantitative detection of mouse OPN in various biological samples. This kit is specifically developed to provide researchers and clinicians with a simple and efficient method for measuring the levels of OPN in mouse serum, plasma, and other biological fluids.
The Mouse OPN ELISA Kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique, which involves the use of two highly specific antibodies that bind to different epitopes of the OPN protein. The first antibody is coated onto the microplate wells, while the second antibody is conjugated to horseradish peroxidase (HRP) and serves as the detection reagent.
The assay procedure is straightforward and can be completed within a short period of time. The samples are first added to the coated microplate wells, followed by the addition of the detection reagent. After a brief incubation period, the unbound components are washed away, and a substrate solution is added to the wells. The HRP enzyme catalyzes the conversion of the substrate into a colored product, which is proportional to the amount of OPN present in the sample.
The Mouse OPN ELISA Kit is highly sensitive, with a detection range of 0.156-10 ng/mL, and has a low limit of detection of 0.078 ng/mL. The kit also exhibits excellent specificity, with minimal cross-reactivity with other related proteins. The results obtained from this kit are highly reproducible and accurate, making it an ideal tool for a wide range of research and clinical applications.
In summary, the Mouse OPN ELISA Kit is a reliable and efficient diagnostic tool that provides accurate and quantitative measurements of mouse OPN levels in various biological samples. Its high sensitivity, specificity, and reproducibility make it an essential tool for researchers and clinicians working in the fields of immunology, oncology, and other related areas.
The Mouse OPN ELISA Kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique, which involves the use of two highly specific antibodies that bind to different epitopes of the OPN protein. The first antibody is coated onto the microplate wells, while the second antibody is conjugated to horseradish peroxidase (HRP) and serves as the detection reagent.
The assay procedure is straightforward and can be completed within a short period of time. The samples are first added to the coated microplate wells, followed by the addition of the detection reagent. After a brief incubation period, the unbound components are washed away, and a substrate solution is added to the wells. The HRP enzyme catalyzes the conversion of the substrate into a colored product, which is proportional to the amount of OPN present in the sample.
The Mouse OPN ELISA Kit is highly sensitive, with a detection range of 0.156-10 ng/mL, and has a low limit of detection of 0.078 ng/mL. The kit also exhibits excellent specificity, with minimal cross-reactivity with other related proteins. The results obtained from this kit are highly reproducible and accurate, making it an ideal tool for a wide range of research and clinical applications.
In summary, the Mouse OPN ELISA Kit is a reliable and efficient diagnostic tool that provides accurate and quantitative measurements of mouse OPN levels in various biological samples. Its high sensitivity, specificity, and reproducibility make it an essential tool for researchers and clinicians working in the fields of immunology, oncology, and other related areas.
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