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SKU:E-EL-M3053
Mouse LH(Luteinizing Hormone) ELISA Kit
Mouse LH(Luteinizing Hormone) ELISA Kit
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$495.00 USD
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The Mouse LH (Luteinizing Hormone) ELISA Kit is a highly reliable and accurate tool designed for the quantitative measurement of Luteinizing Hormone levels in mouse serum, plasma, and other biological samples. This kit utilizes a solid-phase enzyme-linked immunosorbent assay (ELISA) technique, which ensures precise and sensitive detection of LH.
The Mouse LH ELISA Kit is composed of high-quality reagents and materials, including pre-coated microplate wells, LH standards, and detection antibodies. The microplate wells are pre-coated with a specific monoclonal antibody against LH, ensuring high affinity and specificity for LH detection. The LH standards provided in the kit allow for the generation of a standard curve, enabling the accurate quantification of LH levels in the samples.
The assay procedure is straightforward and user-friendly, requiring minimal hands-on time. The samples, along with the provided standards and controls, are added to the microplate wells and incubated. During the incubation, LH present in the samples binds to the immobilized antibody, while unbound substances are washed away. Following this, a biotinylated detection antibody is added, which binds to the captured LH. After another washing step, an enzyme-conjugated streptavidin is added, forming a sandwich complex. The addition of a substrate solution initiates a color reaction, the intensity of which is directly proportional to the amount of LH present in the samples. The reaction is stopped, and the absorbance is measured using a microplate reader at a wavelength of 450 nm.
The Mouse LH ELISA Kit offers a wide dynamic range, allowing for the detection of LH concentrations ranging from 0.156 ng/mL to 10 ng/mL. The sensitivity of the assay is 0.078 ng/mL, ensuring the accurate measurement of even low levels of LH. The kit also provides excellent intra-assay and inter-assay precision, with coefficients of variation less than 10% and 12%, respectively.
This Mouse LH ELISA Kit is an essential tool for researchers and scientists involved in the study of reproductive biology, endocrinology, and fertility. Its high sensitivity, accuracy, and precision make it an indispensable resource for the quantitative analysis of LH levels in mouse samples.
The Mouse LH ELISA Kit is composed of high-quality reagents and materials, including pre-coated microplate wells, LH standards, and detection antibodies. The microplate wells are pre-coated with a specific monoclonal antibody against LH, ensuring high affinity and specificity for LH detection. The LH standards provided in the kit allow for the generation of a standard curve, enabling the accurate quantification of LH levels in the samples.
The assay procedure is straightforward and user-friendly, requiring minimal hands-on time. The samples, along with the provided standards and controls, are added to the microplate wells and incubated. During the incubation, LH present in the samples binds to the immobilized antibody, while unbound substances are washed away. Following this, a biotinylated detection antibody is added, which binds to the captured LH. After another washing step, an enzyme-conjugated streptavidin is added, forming a sandwich complex. The addition of a substrate solution initiates a color reaction, the intensity of which is directly proportional to the amount of LH present in the samples. The reaction is stopped, and the absorbance is measured using a microplate reader at a wavelength of 450 nm.
The Mouse LH ELISA Kit offers a wide dynamic range, allowing for the detection of LH concentrations ranging from 0.156 ng/mL to 10 ng/mL. The sensitivity of the assay is 0.078 ng/mL, ensuring the accurate measurement of even low levels of LH. The kit also provides excellent intra-assay and inter-assay precision, with coefficients of variation less than 10% and 12%, respectively.
This Mouse LH ELISA Kit is an essential tool for researchers and scientists involved in the study of reproductive biology, endocrinology, and fertility. Its high sensitivity, accuracy, and precision make it an indispensable resource for the quantitative analysis of LH levels in mouse samples.
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