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Elabscience

SKU:E-EL-P0002

Porcine IL-1β(Interleukin 1 Beta) ELISA Kit

Porcine IL-1β(Interleukin 1 Beta) ELISA Kit

Regular price $588.00 USD
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The Porcine IL-1β (Interleukin 1 Beta) ELISA Kit is a highly reliable and accurate tool designed for the quantitative detection of Porcine IL-1β in various biological samples. This enzyme-linked immunosorbent assay (ELISA) kit utilizes a solid-phase sandwich format, employing specific antibodies that bind to Porcine IL-1β with high affinity and specificity.

The kit includes all the necessary components for the assay, including pre-coated microplates, standards, controls, and reagents. The microplates are pre-coated with monoclonal antibodies specific to Porcine IL-1β, ensuring optimal capture of the target analyte. The standards provided allow for the generation of a standard curve, enabling the quantification of Porcine IL-1β in the samples.

The assay procedure is straightforward and can be completed within a short period of time. After sample preparation and addition to the microplate wells, the Porcine IL-1β present in the samples binds to the immobilized antibodies. Following a washing step to remove any unbound substances, a biotin-conjugated detection antibody is added, which binds specifically to the captured Porcine IL-1β. This is followed by the addition of an enzyme-conjugated streptavidin, which binds to the biotinylated detection antibody. Finally, a substrate solution is added, resulting in the development of a colored product. The intensity of the color is directly proportional to the concentration of Porcine IL-1β present in the samples, and can be measured spectrophotometrically.

The Porcine IL-1β ELISA Kit offers a wide dynamic range, high sensitivity, and excellent reproducibility. It is suitable for use in various research applications, including the study of inflammation, immune responses, and cytokine regulation in porcine models. The kit provides accurate and precise results, allowing researchers to obtain valuable insights into the role of Porcine IL-1β in various physiological and pathological processes.

In summary, the Porcine IL-1β ELISA Kit is a reliable and efficient tool for the quantitative detection of Porcine IL-1β in biological samples. Its high sensitivity, specificity, and ease of use make it an ideal choice for researchers working in the field of porcine immunology and inflammation.
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