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Elabscience
SKU:E-EL-R0012
Rat IL-1β(Interleukin 1 Beta) ELISA Kit
Rat IL-1β(Interleukin 1 Beta) ELISA Kit
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$495.00 USD
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The Rat IL-1β (Interleukin 1 Beta) ELISA Kit is a highly reliable and accurate tool designed for the quantitative detection of IL-1β in rat samples. This kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique, which ensures high sensitivity and specificity in detecting IL-1β.
The IL-1β ELISA Kit includes all the necessary components for the assay, including pre-coated microplates, standards, detection antibodies, and reagents. The microplates are pre-coated with a monoclonal antibody specific to rat IL-1β, ensuring optimal capture of the target analyte. The detection antibodies are biotinylated and bind specifically to the captured IL-1β, forming a sandwich complex.
The assay procedure is simple and straightforward, with a total assay time of approximately 3 hours. The samples, standards, and controls are added to the microplate wells, followed by the addition of the detection antibodies. After a brief incubation period and washing steps, a streptavidin-HRP conjugate is added, which binds to the biotinylated detection antibodies. Subsequent washing removes any unbound conjugate, and a substrate solution is added to initiate a color reaction. The intensity of the color is directly proportional to the concentration of IL-1β in the samples.
The Rat IL-1β ELISA Kit offers a wide dynamic range, allowing for the accurate quantification of IL-1β in a variety of rat samples, including serum, plasma, and tissue homogenates. The sensitivity of the assay is as low as 0.1 pg/mL, ensuring the detection of even low levels of IL-1β. The kit also provides high precision and reproducibility, with intra-assay and inter-assay coefficients of variation less than 10%.
This ELISA kit is intended for research purposes only and should not be used for diagnostic or therapeutic procedures. It is manufactured in accordance with strict quality control standards to ensure reliable and consistent results. The Rat IL-1β ELISA Kit is an indispensable tool for researchers studying the role of IL-1β in various biological processes and diseases in rats.
The IL-1β ELISA Kit includes all the necessary components for the assay, including pre-coated microplates, standards, detection antibodies, and reagents. The microplates are pre-coated with a monoclonal antibody specific to rat IL-1β, ensuring optimal capture of the target analyte. The detection antibodies are biotinylated and bind specifically to the captured IL-1β, forming a sandwich complex.
The assay procedure is simple and straightforward, with a total assay time of approximately 3 hours. The samples, standards, and controls are added to the microplate wells, followed by the addition of the detection antibodies. After a brief incubation period and washing steps, a streptavidin-HRP conjugate is added, which binds to the biotinylated detection antibodies. Subsequent washing removes any unbound conjugate, and a substrate solution is added to initiate a color reaction. The intensity of the color is directly proportional to the concentration of IL-1β in the samples.
The Rat IL-1β ELISA Kit offers a wide dynamic range, allowing for the accurate quantification of IL-1β in a variety of rat samples, including serum, plasma, and tissue homogenates. The sensitivity of the assay is as low as 0.1 pg/mL, ensuring the detection of even low levels of IL-1β. The kit also provides high precision and reproducibility, with intra-assay and inter-assay coefficients of variation less than 10%.
This ELISA kit is intended for research purposes only and should not be used for diagnostic or therapeutic procedures. It is manufactured in accordance with strict quality control standards to ensure reliable and consistent results. The Rat IL-1β ELISA Kit is an indispensable tool for researchers studying the role of IL-1β in various biological processes and diseases in rats.
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