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Rat CD40L(Cluster Of Differentiation 40 Ligand) ELISA Kit
Rat CD40L(Cluster Of Differentiation 40 Ligand) ELISA Kit
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$609.00 USD
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The Rat CD40L (Cluster of Differentiation 40 Ligand) ELISA Kit is a highly reliable and accurate diagnostic tool designed for the quantitative detection of CD40L in rat serum, plasma, and cell culture supernatants. This kit is specifically designed to provide a sensitive and specific measurement of CD40L levels in rat samples, making it an essential tool for researchers and clinicians alike.
The kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) format, which allows for the detection of CD40L with high sensitivity and specificity. The assay is based on the principle of a solid-phase sandwich ELISA, in which a monoclonal antibody specific to rat CD40L is pre-coated onto a microplate. The sample is then added to the microplate, where it binds to the immobilized antibody. A biotinylated polyclonal antibody specific to rat CD40L is then added, followed by the addition of streptavidin-conjugated horseradish peroxidase (HRP). The resulting complex is then detected using a chromogenic substrate, and the absorbance is measured at a wavelength of 450 nm.
The Rat CD40L ELISA Kit is highly sensitive, with a detection range of 0.156 ng/mL to 10 ng/mL. It is also highly specific, with minimal cross-reactivity with other rat proteins. The kit is easy to use, with a simple and straightforward protocol that can be completed in just a few hours. It is also highly reproducible, with excellent intra- and inter-assay precision.
In summary, the Rat CD40L ELISA Kit is a highly reliable and accurate diagnostic tool that provides a sensitive and specific measurement of CD40L levels in rat samples. It is an essential tool for researchers and clinicians alike, and is ideal for a wide range of applications, including basic research, drug discovery, and clinical diagnostics.
The kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) format, which allows for the detection of CD40L with high sensitivity and specificity. The assay is based on the principle of a solid-phase sandwich ELISA, in which a monoclonal antibody specific to rat CD40L is pre-coated onto a microplate. The sample is then added to the microplate, where it binds to the immobilized antibody. A biotinylated polyclonal antibody specific to rat CD40L is then added, followed by the addition of streptavidin-conjugated horseradish peroxidase (HRP). The resulting complex is then detected using a chromogenic substrate, and the absorbance is measured at a wavelength of 450 nm.
The Rat CD40L ELISA Kit is highly sensitive, with a detection range of 0.156 ng/mL to 10 ng/mL. It is also highly specific, with minimal cross-reactivity with other rat proteins. The kit is easy to use, with a simple and straightforward protocol that can be completed in just a few hours. It is also highly reproducible, with excellent intra- and inter-assay precision.
In summary, the Rat CD40L ELISA Kit is a highly reliable and accurate diagnostic tool that provides a sensitive and specific measurement of CD40L levels in rat samples. It is an essential tool for researchers and clinicians alike, and is ideal for a wide range of applications, including basic research, drug discovery, and clinical diagnostics.
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