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Elabscience
SKU:E-EL-R0558
Rat IL-5(Interleukin 5) ELISA Kit
Rat IL-5(Interleukin 5) ELISA Kit
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$609.00 USD
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The RAT IL-5(INTERLEUKIN 5) ELISA KIT is a highly sensitive and specific assay designed for the quantitative measurement of rat interleukin 5 (IL-5) in serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that recognize different epitopes of the target protein. The assay is easy to perform and provides accurate and reproducible results in a short period of time.
The RAT IL-5(INTERLEUKIN 5) ELISA KIT includes all the necessary components for the assay, including pre-coated microplates, standards, detection antibodies, and streptavidin-HRP conjugate. The microplates are pre-coated with a monoclonal antibody specific to rat IL-5, which captures the target protein from the sample. After washing away unbound materials, a biotinylated polyclonal antibody specific to rat IL-5 is added to the wells, which binds to the captured protein. The streptavidin-HRP conjugate is then added, which binds to the biotinylated antibody. Finally, a substrate solution is added, which reacts with the HRP enzyme to produce a colorimetric signal that is proportional to the amount of rat IL-5 in the sample.
The RAT IL-5(INTERLEUKIN 5) ELISA KIT has a detection range of 15.6-1000 pg/mL and a sensitivity of 7.8 pg/mL. The assay is highly specific for rat IL-5 and does not cross-react with other cytokines or growth factors. The kit is suitable for a wide range of applications, including basic research, drug discovery, and clinical diagnostics. It is compatible with a variety of sample types, including serum, plasma, and cell culture supernatants. The kit is manufactured under strict quality control standards and is validated for accuracy, precision, and reproducibility.
The RAT IL-5(INTERLEUKIN 5) ELISA KIT includes all the necessary components for the assay, including pre-coated microplates, standards, detection antibodies, and streptavidin-HRP conjugate. The microplates are pre-coated with a monoclonal antibody specific to rat IL-5, which captures the target protein from the sample. After washing away unbound materials, a biotinylated polyclonal antibody specific to rat IL-5 is added to the wells, which binds to the captured protein. The streptavidin-HRP conjugate is then added, which binds to the biotinylated antibody. Finally, a substrate solution is added, which reacts with the HRP enzyme to produce a colorimetric signal that is proportional to the amount of rat IL-5 in the sample.
The RAT IL-5(INTERLEUKIN 5) ELISA KIT has a detection range of 15.6-1000 pg/mL and a sensitivity of 7.8 pg/mL. The assay is highly specific for rat IL-5 and does not cross-react with other cytokines or growth factors. The kit is suitable for a wide range of applications, including basic research, drug discovery, and clinical diagnostics. It is compatible with a variety of sample types, including serum, plasma, and cell culture supernatants. The kit is manufactured under strict quality control standards and is validated for accuracy, precision, and reproducibility.
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