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Rat IL-25(Interleukin 25) ELISA Kit
Rat IL-25(Interleukin 25) ELISA Kit
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$609.00 USD
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The RAT IL-25 (Interleukin 25) ELISA Kit is a highly sensitive and specific assay designed to measure the levels of IL-25 in rat serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) technique, which utilizes two specific antibodies that recognize different epitopes of the target protein. The assay is easy to perform and provides accurate and reproducible results in a short period of time.
The kit contains all the necessary components for the assay, including pre-coated microplates, standards, detection antibodies, and streptavidin-HRP conjugate. The assay procedure involves adding the samples and standards to the microplate wells, followed by the addition of the detection antibodies and streptavidin-HRP conjugate. After a washing step, a substrate solution is added, and the reaction is stopped with an acidic solution. The absorbance of each well is measured using a microplate reader, and the concentration of IL-25 in the samples is determined by comparing the absorbance values to the standard curve.
IL-25 is a cytokine that plays a crucial role in the regulation of immune responses and inflammation. It is produced by various cell types, including epithelial cells, T cells, and mast cells, and has been implicated in the pathogenesis of various diseases, such as asthma, allergic rhinitis, and inflammatory bowel disease. The RAT IL-25 ELISA Kit provides a reliable and accurate tool for the quantification of IL-25 levels in rat samples, which can be used for research purposes and for the development of new therapies targeting IL-25.
The kit contains all the necessary components for the assay, including pre-coated microplates, standards, detection antibodies, and streptavidin-HRP conjugate. The assay procedure involves adding the samples and standards to the microplate wells, followed by the addition of the detection antibodies and streptavidin-HRP conjugate. After a washing step, a substrate solution is added, and the reaction is stopped with an acidic solution. The absorbance of each well is measured using a microplate reader, and the concentration of IL-25 in the samples is determined by comparing the absorbance values to the standard curve.
IL-25 is a cytokine that plays a crucial role in the regulation of immune responses and inflammation. It is produced by various cell types, including epithelial cells, T cells, and mast cells, and has been implicated in the pathogenesis of various diseases, such as asthma, allergic rhinitis, and inflammatory bowel disease. The RAT IL-25 ELISA Kit provides a reliable and accurate tool for the quantification of IL-25 levels in rat samples, which can be used for research purposes and for the development of new therapies targeting IL-25.
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