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Elabscience
SKU:E-EL-R2850
Rat ICAM-1/CD54(Intercellular Adhesion Molecule 1) ELISA Kit
Rat ICAM-1/CD54(Intercellular Adhesion Molecule 1) ELISA Kit
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The RAT ICAM-1/CD54 ELISA Kit is a highly sensitive and specific assay designed to measure the levels of intercellular adhesion molecule 1 (ICAM-1) in rat samples. ICAM-1 is a cell surface glycoprotein that plays a crucial role in the immune response by facilitating leukocyte adhesion and migration to sites of inflammation. Elevated levels of ICAM-1 have been associated with various inflammatory diseases, including rheumatoid arthritis, multiple sclerosis, and atherosclerosis.
The kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) format, in which ICAM-1 in the sample is captured by a specific monoclonal antibody coated on the microplate wells. After washing away unbound materials, a biotinylated detection antibody is added, followed by a streptavidin-conjugated horseradish peroxidase (HRP) enzyme. The HRP catalyzes a colorimetric reaction with the substrate, resulting in a measurable signal that is proportional to the amount of ICAM-1 in the sample. The assay has a detection range of 0.156-10 ng/mL and a sensitivity of 0.078 ng/mL.
The RAT ICAM-1/CD54 ELISA Kit is easy to use and provides accurate and reproducible results. It includes all necessary reagents and a detailed protocol, and can be used with a variety of rat sample types, including serum, plasma, and tissue homogenates. The kit is ideal for researchers studying the role of ICAM-1 in inflammation and immune-related diseases, and can also be used for drug discovery and development.
The kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) format, in which ICAM-1 in the sample is captured by a specific monoclonal antibody coated on the microplate wells. After washing away unbound materials, a biotinylated detection antibody is added, followed by a streptavidin-conjugated horseradish peroxidase (HRP) enzyme. The HRP catalyzes a colorimetric reaction with the substrate, resulting in a measurable signal that is proportional to the amount of ICAM-1 in the sample. The assay has a detection range of 0.156-10 ng/mL and a sensitivity of 0.078 ng/mL.
The RAT ICAM-1/CD54 ELISA Kit is easy to use and provides accurate and reproducible results. It includes all necessary reagents and a detailed protocol, and can be used with a variety of rat sample types, including serum, plasma, and tissue homogenates. The kit is ideal for researchers studying the role of ICAM-1 in inflammation and immune-related diseases, and can also be used for drug discovery and development.
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