Human MMP-2(Matrix Metalloproteinase 2) CLIA Kit

The HUMAN MMP-2 CLIA KIT is a highly sensitive and specific assay kit designed for the quantitative measurement of Matrix Metalloproteinase 2 (MMP-2) in human serum, plasma, and cell culture supernatants. MMP-2 is a zinc-dependent endopeptidase that plays a crucial role in the degradation of extracellular matrix (ECM) components, such as collagen and elastin. Dysregulation of MMP-2 has been implicated in various pathological conditions, including cancer, cardiovascular diseases, and inflammatory disorders. Therefore, accurate and reliable measurement of MMP-2 levels is essential for disease diagnosis, prognosis, and therapeutic monitoring.

The HUMAN MMP-2 CLIA KIT utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) format, which involves the use of two highly specific monoclonal antibodies that recognize different epitopes of MMP-2. The first antibody is coated onto the wells of a microtiter plate, where it captures the MMP-2 present in the sample. The second antibody, conjugated to horseradish peroxidase (HRP), is added to the wells, where it binds to the captured MMP-2. The HRP catalyzes the conversion of a colorless substrate into a colored product, which is proportional to the amount of MMP-2 present in the sample. The assay has a detection range of 0.156-10 ng/mL, with a sensitivity of 0.078 ng/mL.

The HUMAN MMP-2 CLIA KIT is easy to use, with a simple and straightforward protocol that can be completed within 3 hours. The kit contains all the necessary reagents and components, including the microtiter plate, the antibodies, the HRP conjugate, the substrate, and the stop solution. The kit also includes a standard curve and a set of controls, which allow for accurate and precise quantification of MMP-2 levels. The assay has been validated for use with a variety of sample types, including serum, plasma, and cell culture supernatants, and has been shown to have excellent intra- and inter-assay precision and accuracy. Overall, the HUMAN MMP-2 CLIA KIT is a reliable and sensitive tool for the measurement of MMP-2 levels in human samples, and is suitable for use in both research and clinical settings.