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SKU:E-UNEL-M0045
Uncoated Mouse IGF-1(Insulin-like Growth Factor 1) ELISA Kit
Uncoated Mouse IGF-1(Insulin-like Growth Factor 1) ELISA Kit
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$399.00 USD
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The Uncoated Mouse IGF-1 (Insulin-like Growth Factor 1) ELISA Kit is a highly reliable and accurate diagnostic tool designed for the quantitative determination of IGF-1 levels in mouse serum, plasma, and other biological fluids. This ELISA kit is uncoated, which means that it does not require any pre-coated plates, making it a cost-effective and efficient solution for laboratory testing.
The kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique, which involves the use of two specific antibodies that bind to different epitopes of the IGF-1 molecule. The first antibody is immobilized on the microplate wells, while the second antibody is conjugated to horseradish peroxidase (HRP). The sample is added to the wells, and the IGF-1 molecules present in the sample bind to the immobilized antibody. The HRP-conjugated antibody is then added, and it binds to the IGF-1 molecules, forming a sandwich complex. The unbound materials are washed away, and a substrate solution is added, which reacts with the HRP enzyme to produce a color change. The intensity of the color is directly proportional to the amount of IGF-1 present in the sample, and it can be measured using a microplate reader.
The Uncoated Mouse IGF-1 ELISA Kit is highly sensitive, with a detection range of 0.156-10 ng/mL, and it has a low limit of detection (LOD) of 0.078 ng/mL. The kit has a high degree of specificity, with minimal cross-reactivity with other related molecules. It is easy to use, with a simple protocol that can be completed in less than 4 hours. The kit is supplied with all the necessary reagents and components, including microplate wells, standards, controls, and buffers, to ensure accurate and reproducible results.
In summary, the Uncoated Mouse IGF-1 ELISA Kit is a reliable and efficient diagnostic tool for the quantitative determination of IGF-1 levels in mouse serum, plasma, and other biological fluids. It is highly sensitive, specific, and easy to use, making it an ideal solution for laboratory testing.
The kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique, which involves the use of two specific antibodies that bind to different epitopes of the IGF-1 molecule. The first antibody is immobilized on the microplate wells, while the second antibody is conjugated to horseradish peroxidase (HRP). The sample is added to the wells, and the IGF-1 molecules present in the sample bind to the immobilized antibody. The HRP-conjugated antibody is then added, and it binds to the IGF-1 molecules, forming a sandwich complex. The unbound materials are washed away, and a substrate solution is added, which reacts with the HRP enzyme to produce a color change. The intensity of the color is directly proportional to the amount of IGF-1 present in the sample, and it can be measured using a microplate reader.
The Uncoated Mouse IGF-1 ELISA Kit is highly sensitive, with a detection range of 0.156-10 ng/mL, and it has a low limit of detection (LOD) of 0.078 ng/mL. The kit has a high degree of specificity, with minimal cross-reactivity with other related molecules. It is easy to use, with a simple protocol that can be completed in less than 4 hours. The kit is supplied with all the necessary reagents and components, including microplate wells, standards, controls, and buffers, to ensure accurate and reproducible results.
In summary, the Uncoated Mouse IGF-1 ELISA Kit is a reliable and efficient diagnostic tool for the quantitative determination of IGF-1 levels in mouse serum, plasma, and other biological fluids. It is highly sensitive, specific, and easy to use, making it an ideal solution for laboratory testing.
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