Uncoated Mouse IL-1α(Interleukin 1 Alpha) ELISA Kit

The UNCOATED MOUSE IL-1Α(INTERLEUKIN 1 ALPHA) ELISA KIT is a highly sensitive and specific assay designed for the quantitative determination of mouse IL-1α in serum, plasma, and cell culture supernatants. This kit is uncoated, meaning that the capture antibody is not pre-coated onto the microplate, allowing for greater flexibility in assay design and optimization. The kit includes all necessary reagents and materials for the assay, including a pre-coated standard curve, detection antibody, streptavidin-HRP conjugate, and TMB substrate solution.

The assay is based on the sandwich ELISA principle, in which a capture antibody is used to immobilize the target protein on the microplate, followed by the addition of a detection antibody and a secondary antibody conjugated to HRP. The resulting colorimetric signal is proportional to the amount of target protein present in the sample. The UNCOATED MOUSE IL-1Α(INTERLEUKIN 1 ALPHA) ELISA KIT has a detection range of 15.6-1000 pg/mL, with a sensitivity of 7.8 pg/mL. The assay has a high degree of accuracy and reproducibility, with intra- and inter-assay coefficients of variation of less than 10%. This kit is ideal for researchers studying the role of IL-1α in inflammation, immune response, and other biological processes in mice.