Uncoated Mouse Cys-C(Cystatin C) ELISA Kit

The Uncoated Mouse Cys-C(Cystatin C) ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of mouse Cystatin C in serum, plasma, and other biological fluids. This kit is uncoated, which means that the capture antibody is not pre-coated onto the microplate, allowing for greater flexibility in assay design and optimization. The kit includes all necessary reagents and materials for the assay, including a pre-coated standard curve, detection antibody, and streptavidin-HRP conjugate.

The assay is based on the sandwich ELISA principle, in which a capture antibody is used to immobilize the target antigen on the microplate, followed by the addition of a detection antibody and a secondary antibody conjugated to horseradish peroxidase (HRP). The HRP catalyzes a colorimetric reaction, which is then measured using a microplate reader. The sensitivity of the assay is 0.1 ng/mL, with a dynamic range of 0.156-10 ng/mL.

Cystatin C is a small protein that is produced by all nucleated cells and is freely filtered by the glomerulus in the kidney. It is a sensitive marker of renal function and is used clinically to estimate glomerular filtration rate (GFR). The Uncoated Mouse Cys-C(Cystatin C) ELISA Kit is a valuable tool for researchers studying kidney function and disease in mouse models, as well as for drug discovery and development. Its high sensitivity and specificity, along with its ease of use and flexibility, make it an ideal choice for a wide range of applications.