Uncoated Human ENG(Endoglin) ELISA Kit

The Uncoated Human ENG (Endoglin) ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of human Endoglin in serum, plasma, and cell culture supernatants. This kit is uncoated, meaning that the capture antibody is not pre-coated onto the microplate, allowing for greater flexibility in assay design and optimization. The kit includes all necessary reagents and components for the assay, including a pre-coated standard curve, detection antibody, streptavidin-HRP conjugate, and TMB substrate solution.

The assay is based on a sandwich ELISA principle, where the capture antibody binds to Endoglin in the sample, and the detection antibody binds to a different epitope on the protein. The streptavidin-HRP conjugate then binds to the detection antibody, and the TMB substrate solution is added to produce a colorimetric signal that is proportional to the amount of Endoglin present in the sample. The assay has a detection range of 31.25-2000 pg/mL, with a sensitivity of 18.75 pg/mL. The kit has been validated for use with human samples, and has a high degree of accuracy and reproducibility. This kit is ideal for researchers studying the role of Endoglin in various disease states, including cancer, cardiovascular disease, and inflammation.