ExKine™ Total Membrane Protein Extraction Kit

The ExKine™ Total Membrane Protein Extraction Kit provides a simple, rapid, and reproducible method to prepare cellular protein fractions highly enriched in membrane and hydrophobic proteins. The sample is first mixed and homogenized in the membrane protein extraction buffers. After a brief incubation at 37°C, the sample is centrifuged, producing an upper aqueous phase and a lower detergent-rich phase. Proteins anchored to the membrane or containing transmembrane domains are efficiently partitioned to the detergent-rich phase.