ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit

The ExKine™ Nuclear and Cytoplasmic Protein Extraction Kit enables stepwise separation and preparation of crude cytoplasmic and nuclear extracts from mammalian cultured cells or tissues. This kit allows cells to swell with hypotonic buffer, followed by disruption, removal of the cytoplasmic fraction, and release of nuclear proteins using a high salt buffer. Non-denatured, active proteins are purified in less than two hours.