NDFIP1 Antibody

Size

100ug

Clone Number

Aliases

0610010M22Rik antibody; Breast cancer associated protein SGA 1M antibody; Breast cancer-associated protein SGA-1M antibody; MGC10924 antibody; N4WBP5 antibody; Ndfip1 antibody; Nedd4 family interacting protein 1 antibody; NEDD4 family-interacting protein 1 antibody; NEDD4 WW domain binding protein 5 antibody; NEDD4 WW domain-binding protein 5 antibody; NFIP1_HUMAN antibody; Putative MAPK activating protein PM13 antibody; Putative MAPK-activating protein PM13 antibody; Putative NF kappa B activating protein 164 antibody; Putative NF-kappa-B-activating protein 164 antibody; Putative NFKB and MAPK activating protein antibody; Putative NFKB and MAPK-activating protein antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q9BT67

Immunogen

Recombinant Human NEDD4 family-interacting protein 1 protein (2-116AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200

Background

Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation (By similarity). In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity (PubMed:19706893). Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate (PubMed:20534535). Enhances the ubiquitination of BRAT1 mediated by E3 ubiquitin-protein ligases: NEDD4, NEDD4L and ITCH, and is required for the nuclear localization of ubiquitinated BRAT1 (PubMed:25631046).

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

NDFIP1

Research Areas

Epigenetics and Nuclear Signaling; Cell biology