NANOG Monoclonal Antibody

Size

100ul

Clone Number

8A1D11

Aliases

Homeobox protein NANOG (Homeobox transcription factor Nanog) (hNanog), NANOG

Immunogen Species

Human

UniProt ID

Q9H9S0

Immunogen

Recombinant Human Homeobox protein NANOG protein (1-305AA)

Raised in

Mouse

Species Reactivity

Human, Mouse, Rat

Tested Applications

ELISA, WB, ICC, IF, FC; Recommended dilution: WB:1:500-1:2000, ICC:1:50-1:500, IF:1:50-1:200

Background

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Clonality

Monoclonal

Isotype

IgG1

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

NANOG

Research Areas

Stem Cells