NANOG Antibody

Size

100ug

Clone Number

Aliases

Embryonic stem cell specific homeobox protein (Nanog) antibody; ENK antibody; FLJ12581 antibody; hNanog antibody; Homeobox protein NANOG antibody; Homeobox transcription factor Nanog antibody; homeobox transcription factor Nanog-delta 48 antibody; NANOG antibody; Nanog homeobox antibody; NANOG_HUMAN antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

Q9H9S0

Immunogen

Recombinant Human Homeobox protein NANOG protein (1-305AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, WB, IHC; Recommended dilution: WB:1:2000-1:5000, IHC:1:20-1:200

Background

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5\\\'-TAAT[GT][GT]-3\\\' or 5\\\'-[CG][GA][CG]C[GC]ATTAN[GC]-3\\\'. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Conjugate

Non-conjugated

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

NANOG

Research Areas

Epigenetics and Nuclear Signaling; Cancer; Developmental biology; Stem cells