N antibody; Nucleoprotein antibody; EC 3.1.13.- antibody; Nucleocapsid protein antibody; Protein N antibody
Immunogen Species
Junin mammarenavirus
UniProt ID
P14239
Immunogen
Recombinant Junin mammarenavirus Nucleoprotein protein (1-564AA)
Raised in
Rabbit
Species Reactivity
Junin mammarenavirus
Tested Applications
ELISA, WB; Recommended dilution: WB:1:500-1:5000
Background
Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Disables the host innate defense by interfering with beta interferon (IFNB) production through the inhibition of host IRF3 phosphorylation and activation by host IKBKE. Through the interaction with host IKBKE, strongly inhibits the phosphorylation and nuclear translocation of IRF3, a protein involved in IFN activation pathway, leading to the inhibition of IFNB and IRF3-dependent promoters activation (By similarity). Interacts with host IKBKE (via Protein kinase domain); the interaction inhibits IKBKE kinase activity.
