MOCS3 Antibody

Size

100ul

Clone Number

Aliases

Adenylyltransferase MOCS3 antibody; dJ914P20.3 antibody; MGC9252 antibody; MOCS 3 antibody; MOCS3 antibody; MOCS3_HUMAN antibody; Molybdenum cofactor synthesis 3 antibody; Molybdenum cofactor synthesis protein 3 antibody; Molybdopterin synthase sulfurylase antibody; Molybdopterin-synthase sulfurtransferase antibody; MPT synthase sulfurylase antibody; Sulfur carrier protein MOCS2A adenylyltransferase antibody; Sulfur carrier protein MOCS2A sulfurtransferase antibody; Sulfurtransferase MOCS3 antibody; UBA 4 antibody; UBA4 ubiquitin activating enzyme E1 homolog antibody; UBA4 antibody

Immunogen Species

Homo sapiens (Human)

UniProt ID

O95396

Immunogen

Recombinant Human Adenylyltransferase and sulfurtransferase MOCS3 protein (271-460AA)

Raised in

Rabbit

Species Reactivity

Human

Tested Applications

ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200

Background

Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Conjugate

Non-conjugated

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Stroage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Names

MOCS3

Research Areas

Metabolism; Signal transduction