Mini Samples ELISA Kit for Thyroxine (T4)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

3.70-300ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 1.34ng/mL

Sample type

Serum, plasma and other biological fluids

Assay length

3h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Thyroxine (T4) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Thyroxine (T4) and unlabeled Mini Samples Thyroxine (T4) (Standards or samples) with the pre-coated antibody specific to Mini Samples Thyroxine (T4). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Thyroxine (T4) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Thyroxine (T4) in the sample.

Alternative Names

3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine

Item Name

Thyroxine

Research Area

Endocrinology;Hormone metabolism;

Reference