Mini Samples ELISA Kit for Quinolinic Acid (QA)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

1.23-100ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.55ng/mL

Sample type

Serum, plasma and other biological fluids

Assay length

3h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Quinolinic Acid (QA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Quinolinic Acid (QA) and unlabeled Mini Samples Quinolinic Acid (QA) (Standards or samples) with the pre-coated antibody specific to Mini Samples Quinolinic Acid (QA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Quinolinic Acid (QA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Quinolinic Acid (QA) in the sample.

Alternative Names

QUIN; Pyridine-2,3-Dicarboxylic Acid

Item Name

Quinolinic Acid

Research Area

Reference