Mini Samples ELISA Kit for Prostaglandin E2 (PGE2)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

24.69-2,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Pan-species (General)

Sensitivity

The minimum detectable dose of this kit is typically less than 9.22pg/mL

Sample type

Serum, plasma and other biological fluids

Assay length

3h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Prostaglandin E2 (PGE2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Prostaglandin E2 (PGE2) and unlabeled Mini Samples Prostaglandin E2 (PGE2) (Standards or samples) with the pre-coated antibody specific to Mini Samples Prostaglandin E2 (PGE2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Prostaglandin E2 (PGE2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Prostaglandin E2 (PGE2) in the sample.

Alternative Names

PG-E2

Item Name

Prostaglandin E2

Research Area

Metabolic pathway;Infection immunity;Endocrinology;Hormone metabolism;

Reference