Mini Samples ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

370.4-30,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 129.4pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Procollagen I N-Terminal Propeptide (PINP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Procollagen I N-Terminal Propeptide (PINP) and unlabeled Mini Samples Procollagen I N-Terminal Propeptide (PINP) (Standards or samples) with the pre-coated antibody specific to Mini Samples Procollagen I N-Terminal Propeptide (PINP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Procollagen I N-Terminal Propeptide (PINP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Procollagen I N-Terminal Propeptide (PINP) in the sample.

Alternative Names

P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide

Item Name

Procollagen I N-Terminal Propeptide

Research Area

Metabolic pathway;Hepatology;Bone metabolism;

Reference