Mini Samples ELISA Kit for Pepsin (PP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

1.23-100ng/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 0.51ng/mL

Sample type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Double-antibody Sandwich

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Pepsin (PP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Pepsin (PP) and unlabeled Mini Samples Pepsin (PP) (Standards or samples) with the pre-coated antibody specific to Mini Samples Pepsin (PP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Pepsin (PP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Pepsin (PP) in the sample.

Alternative Names

Item Name

Pepsin

Research Area

Enzyme & Kinase;Metabolic pathway;Tumor immunity;Gastroenterology;

Reference