Mini Samples ELISA Kit for Parathyroid Hormone (PTH)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

9.88-800pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Mus musculus (Mouse)

Sensitivity

The minimum detectable dose of this kit is typically less than 3.59pg/mL

Sample type

serum, plasma and other biological fluids

Assay length

3h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Parathyroid Hormone (PTH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Parathyroid Hormone (PTH) and unlabeled Mini Samples Parathyroid Hormone (PTH) (Standards or samples) with the pre-coated antibody specific to Mini Samples Parathyroid Hormone (PTH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Parathyroid Hormone (PTH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Parathyroid Hormone (PTH) in the sample.

Alternative Names

iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin

Item Name

Parathyroid Hormone

Research Area

Metabolic pathway;Endocrinology;Hormone metabolism;

Reference