Mini Samples ELISA Kit for Insulin (INS)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

123.5-10,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 49.7pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Insulin (INS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Insulin (INS) and unlabeled Mini Samples Insulin (INS) (Standards or samples) with the pre-coated antibody specific to Mini Samples Insulin (INS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Insulin (INS) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Insulin (INS) in the sample.

Alternative Names

Item Name

Insulin

Research Area

Metabolic pathway;Endocrinology;Hormone metabolism;

Reference