Mini Samples ELISA Kit for Glucagon Like Peptide 1 (GLP1)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

12.35-1,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Canis familiaris; Canine (Dog), Bos taurus; Bovine (Cattle)

Sensitivity

The minimum detectable dose of this kit is typically less than 4.64pg/mL

Sample type

Serum, plasma and other biological fluids

Assay length

3h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Glucagon Like Peptide 1 (GLP1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Glucagon Like Peptide 1 (GLP1) and unlabeled Mini Samples Glucagon Like Peptide 1 (GLP1) (Standards or samples) with the pre-coated antibody specific to Mini Samples Glucagon Like Peptide 1 (GLP1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Glucagon Like Peptide 1 (GLP1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Glucagon Like Peptide 1 (GLP1) in the sample.

Alternative Names

Item Name

Glucagon Like Peptide 1

Research Area

Endocrinology;Cardiovascular biology;Hepatology;Hormone metabolism;

Reference