Mini Samples ELISA Kit for Gastric Inhibitory Polypeptide (GIP)

Format

48T, 96T, 96T×5, 96T×10, 96T×100

Detection range

61.7-5,000pg/mL

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species

Rattus norvegicus (Rat)

Sensitivity

The minimum detectable dose of this kit is typically less than 21.9pg/mL

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay length

3h

Method

Competitive Inhibition

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Gastric Inhibitory Polypeptide (GIP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Gastric Inhibitory Polypeptide (GIP) and unlabeled Mini Samples Gastric Inhibitory Polypeptide (GIP) (Standards or samples) with the pre-coated antibody specific to Mini Samples Gastric Inhibitory Polypeptide (GIP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Gastric Inhibitory Polypeptide (GIP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Gastric Inhibitory Polypeptide (GIP) in the sample.

Alternative Names

Incretin hormone; Glucose Dependent Insulinotropic Peptide

Item Name

Gastric Inhibitory Polypeptide

Research Area

Endocrinology;Gastroenterology;Hormone metabolism;

Reference